Search results for "cell lo"

showing 8 items of 8 documents

Comparison of DMEK and DSAEK in Eyes With Endothelial Decompensation After Previous Penetrating Keratoplasty.

2021

PURPOSE Posterior lamellar keratoplasty is increasingly applied in patients with endothelial decompensation after penetrating keratoplasty (PK). The aim of this study was to compare the results of Descemet membrane endothelial keratoplasty (DMEK) and Descemet stripping automated endothelial keratoplasty (DSAEK) after PK. METHODS In this retrospective study, clinical data of 30 patients who received DMEK (n = 19) or DSAEK (n = 11) for endothelial decompensation after PK were evaluated. All lamellar keratoplasties were performed at the Department of Ophthalmology at University Hospital Mainz, Germany. Primary end point included best-corrected visual acuity, and secondary end points included e…

AdultMalemedicine.medical_specialtyVisual acuityDescemet membraneVisual AcuityCell CountCorneal DiseasesYoung AdultOphthalmologymedicineHumansDecompensationIn patientPostoperative PeriodAgedRetrospective StudiesAged 80 and overGraft rejectionbusiness.industryGraft SurvivalEndothelial CellsCorneal Endothelial Cell LossMiddle AgedUniversity hospitalDescemet stripping automated endothelial keratoplastyEndothelial cell densityOphthalmologyFemalemedicine.symptombusinessDescemet Stripping Endothelial KeratoplastyKeratoplasty PenetratingCornea
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Short-Time Ocular Ischemia Induces Vascular Endothelial Dysfunction and Ganglion Cell Loss in the Pig Retina

2019

Visual impairment and blindness are often caused by retinal ischemia-reperfusion (I/R) injury. We aimed to characterize a new model of I/R in pigs, in which the intraocular pathways were not manipulated by invasive methods on the ocular system. After 12 min of ischemia followed by 20 h of reperfusion, reactivity of retinal arterioles was measured in vitro by video microscopy. Dihydroethidium (DHE) staining, qPCR, immunohistochemistry, quantification of neurons in the retinal ganglion cell layer, and histological examination was performed. Retinal arterioles of I/R-treated pigs displayed marked attenuation in response to the endothelium-dependent vasodilator, bradykinin, compared to sham-tre…

Retinal Ganglion CellsVascular Endothelial Growth Factor A0301 basic medicinePathologySwineNitric Oxide Synthase Type IIVasodilationendothelial dysfunctionlcsh:Chemistrychemistry.chemical_compound0302 clinical medicineIschemiaEndothelial dysfunctionlcsh:QH301-705.5SpectroscopyGeneral MedicineComputer Science ApplicationsArteriolesmedicine.anatomical_structureRetinal ganglion cellReperfusion InjuryNADPH Oxidase 2medicine.medical_specialtyEndotheliumRetinal ArteryI/R injuryIschemiaretinal arteriolesBradykininRetinal ganglionRetinaArticleCatalysisganglion cell lossInorganic Chemistry03 medical and health sciencesmedicineAnimalsPhysical and Theoretical ChemistryMolecular BiologyRetinabusiness.industryOrganic ChemistryRetinalHypoxia-Inducible Factor 1 alpha Subunitmedicine.disease030104 developmental biologylcsh:Biology (General)lcsh:QD1-999chemistry030221 ophthalmology & optometryEndothelium VascularReactive Oxygen SpeciesbusinessInternational Journal of Molecular Sciences
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Effects of LASIK on Corneal Endothelium Using the 15-kHz IntraLase Femtosecond Laser

2011

PURPOSE: To assess the effects of LASIK with the 15-kHz IntraLase femtosecond laser on corneal endothelium. METHODS: In a prospective, single-center clinical trial, 138 patients (mean age: 32.0±7.1 years [range: 21 to 42 years]) underwent femtosecond LASIK for the correction of myopia −0.75 to −9.00 diopters (D) and cylinder up to 3.25 D. Patients were divided into two groups: contact lens group (n=76) and non-contact lens group (n=62). Pre- and 12-month postoperative specular microscopy of the central corneal endothelium was performed. The integrity of the central endothelium was assessed in terms of endothelial cell density and percentage of hexagonality. RESULTS: In the contact lens gro…

Adultmedicine.medical_specialtyCorneal endotheliumgenetic structuresEndotheliummedicine.medical_treatmentKeratomileusis Laser In SituVisual AcuityCell CountKeratomileusisRefraction OcularYoung AdultOphthalmologyMyopiamedicineHumansProspective StudiesDioptrebusiness.industryEndothelium CornealLASIKCorneal Endothelial Cell LossContact Lenses Hydrophiliceye diseasesSurgeryContact lensOphthalmologymedicine.anatomical_structureLens (anatomy)FemtosecondLasers ExcimerSurgerysense organsbusinessJournal of Refractive Surgery
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Endothelial cell loss after toric iris-fixated phakic intraocular lens implantation: three-year follow-up.

2007

ABSTRACT PURPOSE: To study quantitative changes in endothelial cell count after implantation of the toric iris-fixated phakic intraocular lens. METHODS: A prospective, non-randomized, self-controlled clinical trial was conducted of 40 eyes (28 myopic, 12 hyperopic) of 23 patients with high ametropia and astigmatism. Non-contact computer-assisted endothelial microscopy was performed before and I1 2, and 3 years after surgery. RESULTS: In the myopic group, mean preoperative endothelial cell count was 3179±531 cells/mm p 2 (range: 1800 to 3900 cells/mm2). The mean intraindividual endothelial cell loss was -1.83±2.25% (range: -9.09% to 0%) in the first year; -1.83+2.95% (range: -7.74% to 3.80%)…

AdultMalemedicine.medical_specialtyTime FactorsIrisCell CountAstigmatismPhakic intraocular lensEndothelial cell countCorneal DiseasesPostoperative ComplicationsLens Implantation IntraocularOphthalmologyLens CrystallineMedicineHumansProspective Studiesbusiness.industryEndothelium CornealSuture TechniquesMiddle Agedmedicine.diseaseCell lossRefractive Surgical ProceduresEndothelial stem cellOphthalmologySurgeryFemalebusinessFollow-Up StudiesJournal of refractive surgery (Thorofare, N.J. : 1995)
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Clinical outcomes after implantation of a posterior chamber collagen copolymer phakic intraocular lens with a central hole for myopic correction.

2013

Purpose To evaluate the efficacy, predictability, safety, and stability of a new-model posterior chamber Implantable Collamer Lens phakic intraocular lens (pIOL) (V4c Visian) to correct myopia. Setting Private practice, Oviedo, Spain. Design Case series. Methods This study enrolled patients who had implantation of a new pIOL design with a central hole for more natural aqueous flow, eliminating the need for neodymium:YAG iridotomy or peripheral iridectomy. The uncorrected (UDVA) and corrected (CDVA) distance visual acuities, refractive error, intraocular pressure (IOP), endothelial cell count, central vault, and adverse events were evaluated 6 months postoperatively. Results The study enroll…

AdultMalemedicine.medical_specialtyRefractive errorIntraocular pressurePhakic Intraocular Lensesgenetic structuresmedicine.medical_treatmentVisual AcuityCell CountPhakic intraocular lensProsthesis DesignYoung AdultLens Implantation IntraocularmedicineMyopiaHumansProspective StudiesProspective cohort studyDioptreIntraocular PressureAqueous flowbusiness.industryEndothelium CornealCorneal Endothelial Cell Lossmedicine.diseaseRefractive Errorseye diseasesSensory SystemsSurgeryOphthalmologyTreatment OutcomePrivate practiceIridectomySurgeryFemalesense organsCollagenbusinessFollow-Up StudiesJournal of cataract and refractive surgery
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Translating intracarotid artery transplantation of bone marrow-derived NCS-01 cells for ischemic stroke: Behavioral and histological readouts and mec…

2019

Abstract The present study used in vitro and in vivo stroke models to demonstrate the safety, efficacy, and mechanism of action of adult human bone marrow‐derived NCS‐01 cells. Coculture with NCS‐01 cells protected primary rat cortical cells or human neural progenitor cells from oxygen glucose deprivation. Adult rats that were subjected to middle cerebral artery occlusion, transiently or permanently, and subsequently received intracarotid artery or intravenous transplants of NCS‐01 cells displayed dose‐dependent improvements in motor and neurological behaviors, and reductions in infarct area and peri‐infarct cell loss, much better than intravenous administration. The optimal dose was 7.5 × …

Male0301 basic medicinecell lofunctional recoverymedicine.medical_treatmentBasic fibroblast growth factorCell- and Tissue-Based TherapyPharmacologycerebral ischemia03 medical and health scienceschemistry.chemical_compound0302 clinical medicineBone Marrowmental disordersmedicinecytokineAnimalsHumansinfarctcell losslcsh:QH573-671cell transplantationStrokeIschemic Strokelcsh:R5-920business.industrylcsh:CytologyMesenchymal stem cellCell BiologyGeneral MedicineStem-cell therapymedicine.diseaseNeural stem cellcytokinesRatsTransplantation030104 developmental biologymedicine.anatomical_structurechemistrymotor deficitsEnabling Technologies for Cell‐based Clinical TranslationBone marrowStem cellbusinesslcsh:Medicine (General)030217 neurology & neurosurgeryStem Cell TransplantationDevelopmental Biology
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Lentes de contato corneoesclerais adaptadas em córneas irregulares após cirurgia LASIK

2018

ABSTRACT Purpose: We aimed to describe the outcomes of corneoscleral contact lens fitting with multi-aspherical geometry designs in patients with irregular corneas after laser-assisted in situ keratomileusis (LASIK). Methods: This was a retrospective series of 18 patients (age, 26-38 years) selected from an eye clinic database. Participants were required to have unsatisfactory visual acuity with their current contact lenses or spectacles after LASIK for myopia correction. All patients were fitted with corneoscleral contact lenses designed to correct corneal surface irregularities. A diagnostic trial set was used for fitting, and assessments were performed according to a standardized methodo…

AdultCorneal surgery laserIntraocular pressuremedicine.medical_specialtyCorneal Wavefront AberrationVisual acuityPaquimetria corneanagenetic structuresIntraocular pressuremedicine.medical_treatmentKeratomileusis Laser In SituVisual AcuityLentes de contatoKeratomileusisCorneal endothelial cell losslaw.invention03 medical and health sciencesCorneal pachymetry0302 clinical medicinePerda de células endoteliais da córneaPressão intraocularlcsh:OphthalmologylawOphthalmologyMyopiaHumansMedicineContact lensesCorneal pachymetryCirurgia da córnea a laserRetrospective Studiesmedicine.diagnostic_testbusiness.industryCorneal TopographyLASIKGeneral MedicineContact Lenses HydrophilicCorneal topographyeye diseasesLens (optics)Contact lensOphthalmologylcsh:RE1-994030221 ophthalmology & optometrysense organsmedicine.symptombusiness030217 neurology & neurosurgeryFollow-Up Studies
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AQUEOUS EXTRACTS FROM LEAVES AND RHIZOMES OF THE MARINE SEAGRASS POSIDONIA OCEANICA EXHIBIT ANTI-LIVER CANCER ABILITY IN VITRO

2022

cancer cells Poseidonia oceanica leaf extract rhizome extract cell viability cell cycle apoptosis autophagy cell locomotionSettore BIO/05 - ZoologiaSettore BIO/06 - Anatomia Comparata E Citologia
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